1846. Buster Bunny and Babs Bunny

Buster Bunny (voiced by Charlie Adler and later John Kassir) is the leader of the Tiny Toons gang, a street-smart, blue and white male rabbit who has to deal with the challenges of a tough school curriculum, the machinations of rich kid and local bully Montana Max, and the advances of crazed and ditzy animal lover Elmyra Duff. Like Babs, he will do anything for a laugh, though he is marginally more sane and calm than his female counterpart.

Buster's parents, like many of the main characters on the show, have never been seen on screen. It is known that Buster does live with his mother however, because in Tiny Toons commercials, she has been referenced and has even been spoken to on the phone, mostly as a way to tell Buster that she was on her way home when he was having a party.

Buster has an extreme fondness for Bugs to the point of often imitating his mentor, such as using Bugs' "slap dance" technique from Bully For Bugs (though with considerably less success). Although cool and collected for the most part, there are instances where other characters will get the better of Buster, or some unfortunate mishap will befall him, unlike his mentor Bugs, who for the most part was always on top of his game.

Barbara Ann "Babs" Bunny (voiced by Tress MacNeille) is the lavender pink female rabbit. She is a clown who will do nearly anything for a laugh. She is good at improvisational comedy, and is a comedic impressionist in the vein of Robin Williams. Babs has impersonated or parodied many different actors and fictional characters, including Joan Rivers, Jessica Rabbit,Mae West, Elmer Fudd, Pete Puma, Groucho Marx, Wonder Woman and Madonna. In "Born To Be Riled", her friends become annoyed with her accurate but unflattering impersonations of them and they retaliate by impersonating her in turn. Like Buster, Babs is usually willing to go out of her way for her friends, such as when she sabotages a troupe of swan ballet dancers who are trying to thwart Shirley's debut. She often does quick costume changes, usually achieved by spinning quickly in a circle. Babs also can speak in a deep, sultry voice, often used to seduce Buster. She distinctly hates being called "Barbara Ann", preferring just "Babs".